![]() feltiae E76-S isolate in the Petri dish tests. ipsilon larval population were 52 IJs and 129 IJs, respectively, for S. The lethal concentration values (LC 50 and LC 90) of the A. The highest mortality rate (90%) was obtained by Steinernema carpocapsae E76-S isolate at a concentration of 100 IJs/larva/Petri on the fourth day after inoculation, in the Petri dish experiment. indica 216-H isolates at concentrations of 50 and 100 IJs/cm 2, in the plastic container experiment. ![]() The maximum mortality rate (100%) was reached within 2 days after inoculation, inoculating the Heterorhabditis bacteriophora FLH-4-H and H. ipsilon were recorded first, second, third, and fourth day post inoculation where the mortality rates increased by increasing the concentrations. ipsilon was evaluated at different concentrations (10, 25, 50, 100 IJs/larva/Petri dish and 25, 50, 100 IJs/cm 2 soil) in two different experiment environments including filter papers in Petri dishes and soil in plastic containers under laboratory conditions at 25 ± 1 ☌. In the present study, the efficacy of local EPNs isolates against the fourth larval instar of A. EPNs have been long used for suppressing the soil-dwelling insects like cutworms and are successful biological control agent against A. The black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) is considered to be one of the serious polyphagous pests that spend a large part of its life in the soil environment, where many microorganisms live including entomopathogenic nematodes (EPNs) (Rhabditida: Steinernematidae and Heterorhabditidae).
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